[identity profile] agloriousday.livejournal.com posting in [community profile] craftgrrl

Today I extracted my DNA and put it in a vial to wear around my neck. It's super-easy, anyone can do it. Here are simple instructions I found online: http://www.vampirewear.com/display_page.php?i=18

Here are some suggestions from the things I've learned in two different DNA-extraction workshops and a sequencing lab:

You will have 4 containers (test tube or something with a tight lid for your main DNA container [A]):
A. 3 ml of a sports drink for swooshing (with electrolytes).
B. 2 ml of lysis buffer (0.5 ml cheap/clear liquid detergent and 1.5 ml salted distilled water [with a pinch of salt]).
C. 5 ml of ICED isopropyl alcohol (over 90%) or ethanol (95%).
D. 5 drops of enzyme (can be meat tenderizer, pineapple juice, or contact lens cleaning solution).


1. Swish 3 ml of a sports drink in your mouth vigorously for 30 seconds. Bite gently at your cheeks, not enough to draw blood but enough to slough off as many cheek cells as possible.
2. Spit the solution into container A.
3. Add the lysis solution.
4. Close the lid and gently mix this solution by inverting the container 5 times upside down and right-side up: this will avoid creating too many bubbles and the DNA molecules won't get damaged. The soap solution breaks the cell membranes and the nuclear envelope that are made up of fats - just like soap breaks down grease on your dishes.
5. Open the container and add 5 drops of the enzyme to it, "mix" the solution again for about a minute using the same method as in #4.
6. Fill a cup with hot water from the sink (~50*C) and let container A sit in hot water for 10 minutes.
7. Take it out.
8. Tilt the clear container with your spit and soap solution and gently pour 2-3 teaspoons of the ice cold alcohol down the side of the container so that it forms a layer on the top. DO NOT MIX THIS. If you have a pipette, use the pipette to shoot the alcohol down the side of the container. This step will extract more DNA than not mixing the layers at all, but you may not shake the container yourself.
9. Let the container stand for a few minutes. The white snot that appears in the middle somewhere is your DNA, watch it come up with the bubbles!
10. Use a clean pipette to transfer the DNA booger in the alcohol that it is now in into a clean vial.
11. Stopper and seal the vial with superglue.

VOILA!

It's pretty creepy and nerdy at the same time. There are a million different creepy possibilities with miniature bottles of DNA, just think about it. For example, look at the prices for these pet DNA necklaces, I think they're a bit ridiculous for something so easily done at home, no? Or put some dye in the vial and you've got yourself something that looks like blood, but is 80 times nerdier and more stable.

When I got super-excited about this little project and started looking for resources online, I realized that I really want to make a beaded DNA double-helix necklace. I CAN'T FIND ANY PATTERNS THAT DON'T SUCK. Can anyone help me make this?
I suck at beading, so I have no idea how to do it.

ALSO, I will be a brain-eating amoeba for Halloween. I bought some crazy-looking fabric and a brain that will grow to human-size and human-weight in a bucket of water over the next week, but I can't figure out how to turn the fabric into a costume of a "blob." Can anyone help me with that?

Thanks a lot ahead of time <3

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